METHOD FOR THE MANUFACTURE OF GRADIENT GELS AND METHOD FOR ANALYSING BIOMOLECULES ON ULTRA-LONG IPG GELS

摘要

The invention provides a method for the manufacture of gradient gels, in particular IPG gels, by use of a very dense gel precursor solution, i.e. casting solutions having a density of at least 1.15 kg/L, as well as the polyacrylamide gels as such. The invention also provides a electrophoresis chamber in which a drum carrying a pH-gradient strip is arranged. Furthermore, the invention provides a method of separating a protein mixture in which a moderate voltage gradient is applied.