| 发明名称 |
IMPROVED TRANSFECTION OF EUKARYOTIC CELLS WITH LINEAR POLYNUCLEOTIDES BY ELECTROPORATION |
| 摘要 |
The present invention provides an improved method for gene delivery in eukaryotic cells by electroporation, preferably in human hematopoietic cells, particular dendritic cells. The method of the invention is superior to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for gene delivery, including tumor antigen loading of dendritic cells. |
| 申请公布号 |
WO03000907(A2) |
申请公布日期 |
2003.01.03 |
| 申请号 |
WO2002EP06897 |
申请日期 |
2002.06.21 |
| 申请人 |
SCHULER, GEROLD;N.V. ANTWERPES INNOVATIECENTRUM |
发明人 |
SCHULER, GEROLD;BERNEMAN, ZWI, N.;VAN TENDELOO, VIGGO, F.I.;PONSAERTS, PETER;STROBEL, ISOLDE |
| 分类号 |
C12N15/09;A61K38/00;A61K39/00;A61K48/00;A61P35/00;A61P37/02;C12N5/10;C12N15/87;C12P21/02;C12Q1/02;C12Q1/68 |
主分类号 |
C12N15/09 |
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