METHOD OF ANALYZING PROTEIN OCCURRING IN CELL OR SUBSTANCE INTERACTING WITH THE PROTEIN
摘要
A method which comprises: bringing cells having a protein into contact with a substance interacting with the protein to thereby make them to interact; purifying the cells as being under the interaction with the substance; irradiating the purified cells with laser to thereby directly ionize the substance; and analyzing the thus ionized substance by mass spectrometry. Using this method, the molecular weight, molecular chemical species and molecular structure of a protein occurring in cell surface layer or in cells or a substance interacting with the protein can be easily identified or analyzed within a short time. The above method is a novel method of screening a substance interacting with a protein occurring in cells without resort to the conventional bioassay methods. Also, it is a novel method of judging the presence of a specific protein in cells with the use of a substance interacting with the protein. Moreover, these methods can be conveniently performed and enable accurate and quick judgment.