SEQUENCE-DEPENDENT GENE SORTING TECHNIQUES

摘要

This invention provides a method of sorting genes comprising: (1) preparing ds cDNA molecules from mRNA molecules; (2) digesting the ds cDNA molecules; (3) ligating to the digested cDNA molecules a set of dsDNA oligonucleotide adaptors; (4) amplifying the ligated cDNA molecules; and (5) sorting the amplified cDNA molecules into non-redundant groups. This invention also provides two additional methods of sorting genes. This invention further provides a method of making sub-libraries of ligation sets and a method of making sub-libraries of genetic vectors.