METHOD FOR THE RECOMBINANT PRODUCTION OF PEPTIDIC ANTIVIRAL FUSION INHIBITORS, AND ACETYLATION OF GB41 FRAGMENTS

摘要

A process for the production of an antifusogenic peptide of a length of abou t 10 to 50 amino acids in a prokaryotic host cell, characterized in that, unde r such conditions that inclusion bodies of said non-fusion antifusogenic pepti de or said fusion peptide are formed, a) in said host cell there is expressed a nucleic acid encoding said antifusogenic peptide as a non-fusion peptide or encoding a fusion peptide of a length of about 14 to 70 amino acids consisti ng of said antifusogenic peptide N-terminally linked to a further peptide of a length of about 4 to 30 amino acids; b) said host cell is cultivated; c) sai d inclusion bodies are recovered and solubilized; d) in the case of said fusio n peptide said antifusogenic peptide is cleaved off from said further peptide; and e) said antifusogenic peptide is isolated.