EXPRESSION AND SECRETION VECTOR FOR HUMAN INTERFERON ALPHA AND PROCESS FOR PRODUCING HUMAN INTERFERON ALPHA BY EMPLOYING SAME

摘要

Disclosed in this invention are: an expression vector for the secretive production of human interferon alpha (hIFN alpha ) comprising a polynucleotide encoding a modified E. coli thermostable enterotoxin II signal sequence and a polynucleotide encoding hIFN alpha ) ligated to the 3'-end thereof; a microorganism transformed with the expression vector; and a process for secretively producing human interferon by culturing the microorganism, said process being capable of secreting a soluble form of active hIFN alpha ), which does not contain an additional methionine residue at its N-terminal, into the periplasm of an E. coli cell.