发明名称 |
GENOME WALKING BY SELECTIVE AMPLIFICATION OF NICK-TRANSLATE DNA LIBRARY AND AMPLIFICATION FROM COMPLEX MIXTURES OF TEMPLATES |
摘要 |
Improved methods and reagents for chromosome walking of nucleic acid are discussed herein. A library of amplifiable nick translation molecules is generated, and a chromosome walk is initiated from a known sequence in the nucleic acid by producing at least one nick translate molecule, sequencing part of the nick translate molecule, and producing a second nick translate molecule by initiating the primer extension from the region of the obtained sequence of the prior nick translate molecule.
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申请公布号 |
WO02103054(A1) |
申请公布日期 |
2002.12.27 |
申请号 |
WO2001US44970 |
申请日期 |
2001.11.15 |
申请人 |
RUBICON GENOMICS INC.;MAKAROV, VLADIMIR, L.;KAMBEROV, EMMANUEL;SLEPTSOVA, IRINA |
发明人 |
MAKAROV, VLADIMIR, L.;KAMBEROV, EMMANUEL;SLEPTSOVA, IRINA |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
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