| 摘要 |
Microvolume liquid dispensers that provide simple and inexpensive approaches to making cytology microarrays. In some embodiments, the dispensers comprise tips that comprise an outer sleeve, typically shaped like a funnel, that hol ds a reciprocating needle or pin. The tip of the pin slightly extends beyond th e distal opening of the outer sleeve in one position, and is retracted in another position. When the pin is in the distal position the pin contacts th e inner surfaces of the sleeve and blocks cytology liquid from flowing through the opening of the sleeve. Thus the pin and sleeve cooperate to form a reservoir behind the blockage. When the pin is pushed up into the sleeve, fo r example by touching the tip to a glass slide, a passage is formed between th e outer surface of the pin and the inner surface of the sleeve. The liquid in the reservoir then flows through the passage and onto the slide. Removing th e tip from the substrate moves the pin back to its original position, re-formi ng the reservoir and leaving a predetermined microvolume amount of the liquid o n the slide. |
