Diagnostic phenotype assay for engineered cells and tissues

摘要

The present invention provides an improved method for assessing, monitoring and/or determining the phenotype of cells and tissues. One aspect of the present invention is a method of fabricating phenotype specific gene (PSGs) and house keeping gene (HKGs) targets onto a microarray. Another aspect of the present invention provides a composition containing PSGs and HKGs as targets for high throughput assays including microarray analyses. Another aspect of the present invention is accessing, monitoring and/or determining the phenotype of tissue engineered cells derived from stem cells including embryonic stem cells, embryonic germ cells, fetal stem cells and adult stem cells by hybridizing cDNA probes to either PSG or HKG targets. These methods employ at least 25 PSG targets and no greater than 5000 HKG targets.