| 摘要 |
In a bio-separation system, the detection zone for optical detection of sample analytes is located at a widened zone along the separation channel. In one embodiment of the present invention, the widened detection zone is a micro-bore collar having a micro-channel that coaxially surrounds the exit of a capillary column that defines a capillary channel. A separation support medium including a running buffer fills the capillary column and the collar. In another aspect of the present invention, incident radiation for detection of separated analytes is directed at the detection zone and/or emitted radiation is collected axially along the separation medium, instead of through the boundary walls of the detection zone. In a further aspect of the present invention, the optical detection configuration may be scaled up and implemented in a multi-channel CE system that comprises multiple capillary separation channels.
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