EXTRACT OF ATHYRIUM NIPONICUM HAVING ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES

摘要

PURPOSE: An extract of Athyrium niponicum having antimicrobial and antioxidant activities is provided, thereby obtaining natural antimicrobial agent and antioxidant having improved activities. CONSTITUTION: The extract of Athyrium niponicum having antimicrobial and antioxidant activities is produced by the steps of: adding 6 liter of ethanol into 600 g of dried Athyrium niponicum and suspending them for 48 hours; filtering the suspension with a filter to obtain an ethanol fraction; adding 6 liter of ethanol into the remaining solution to obtain an additional ethanol fraction; concentrating the ethanol fraction under reduced pressure and freeze-drying the concentrate; dissolving the freeze-dried ethanol extract into 500 milliliter of a mixed solution of water and ethanol in the volume ratio of 9:1; adding 500 milliliter of chloroform into the ethanol extract-containing solution to obtain a chloroform extract; sequentially adding ethylacetate and butanol into the chloroform extract to obtain fractions of ethylacetate, butanol and water; and filtering and concentrating them under reduced pressure and freeze-drying the concentrates.