| 摘要 |
<p>Disclosed herein is a novel isolated from nomocotyledon plant sources such as rice bran having the following properties: a) isolated enzyme is a glycoprotein; b) isolated enzyme is stable and catalytically active at high temperatures; c) isolated enzyme is stable and catalytically active at alkaline pH; d) isolated enzyme has molecular weight range of 9 to 12-kDa; e) isolated enzyme is inhibited by inhibitors of serine proteases such as Diisopropylfluorophosphate; f) Isolated enzyme that can utilize both triacylglycerol and phospholipids. This invention also relates to a process of purifying the lipase described above using the following steps: a) delipidation of the plant extract using organic solvents; b) Filtration of the delipidated extract on a suitable matrix; c) Purification of the enzyme using hydrophobic column matrices; d) characterization of the purified product by known methods.</p> |
