| 摘要 |
The invention relates to kits and methods for the sensitive and rapid typing and enumeration of mircoorganisms in a sample. The basic method comprises adhering specific capture-antibodies to a solid support, to bind to microorganisms specific for the antibody, and adding primary antibodies specific to a viability marker of the microorganisms. This is followed by th e addition of secondary antibodies that may be conjugated to a reporter molecule. Preferably the reporter function involves light and the detectable marker is aequorin and the need for a second antibody is overcome. The invention is useful for the detection of a number of different microorganism s including bacteria, fungi and protozoan of a variety of species.
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