| 摘要 |
A liquid chromatograph, an analyzing system and a gradient eluting method contrived such that a high accuracy gradient variation of eluate is enabled and high sensitivity detection or analysis can be realized. The liquid chromatograph comprises means (1) for feeding eluate E at a specified flow rate, means (9) for injecting a sample Sm into the elutriant E, and a column means (13) for separating a soute So contained in the sample Sm. The liquid chromatograph further comprises means (8) for branching the channel (3) of eluate E fed from the feeding means (1) to form branch channels (7, 7, ...) of a specified capacity, storing eluates E1, E2, ... of different composition temporarily in the branch channels (7, 7, ...) and selecting the temporarily stored eluates E1, E2, ..., and means for injecting the sample Sm into a selected eluate E1, E2, ..., feeding it to the separation column (13) and gradient eluting the soute So. An analyzing system where the liquid chromatograph is connected with a mass spectrometer, and a gradient eluting method employing the liquid chromatograph are also provided.
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