发明名称 |
LANDSCAPED ANTIBODIES AND ANTIBODY FRAGMENTS FOR CLINCAL USE |
摘要 |
A method of making a glycosylated antibody or antibody fragment having a reactive ketone group on the glycosylated site is provided. The method comprises expressing a cell transfected with a vector encoding an antibody having one or more glycosylation sites in a culture medium comprising a ketone derivative of a saccharide or saccharide precursor and, in the case of an antibody fragment, fragmenting the resulting antibody into an antigen-binding antibody fragment. Methods of making immunoconjugates comprising the glycosylated antibodies or antibody fragments also are provided, wherein the antibody or antibody fragment is reacted with an agent comprising a ketone-reactive group. Glycosylated antibodies and antibody fragments having a reactive ketone group on the glycosylated site, immunoconjugates comprising such antibodies and antibody fragments and in vivo targeting methods using such antibodies, antibody fragments and immunoconjugates also are provided.
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申请公布号 |
US2002193572(A1) |
申请公布日期 |
2002.12.19 |
申请号 |
US19980185607 |
申请日期 |
1998.11.04 |
申请人 |
LEUNG SHUI-ON;MCBRIDE WILLIAM J.;QU ZHENGXING;HANSEN HANS |
发明人 |
LEUNG SHUI-ON;MCBRIDE WILLIAM J.;QU ZHENGXING;HANSEN HANS |
分类号 |
G01N33/53;A61K39/395;A61K47/48;A61K49/00;C07K16/00;C07K16/28;C12N5/00;C12N15/09;C12P21/02;C12P21/04;C12P21/08;G01N33/531;G01N33/532;(IPC1-7):C12P21/04;C07K2/00;C07K4/00;C07K5/00;C07K7/00;C07K14/00;C07K17/00;A61K38/00 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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