ORGANELLAR PROTEOMICS INCLUDING DETERMINATION OF TARGETS FOR THERAPEUTIC INTERVENTION IDENTIFIED IN THE MITOCHONDRIAL PROTEOME USING MASS SPECTROMETRY

摘要

Mitochondrial targets for drug screening assays and for therapeutic intervention in the treatment of diseases associated with altered mitochondrial function are provided by generating a high-resolution (2-D) map of mitochondrial proteins, and then isolating at least one protein and subjecting it to mass spectrometric analysis, including MALDI-TOF MS. Complete amino acid sequences [SEQ ID NOS:1-8] of polypeptides that comprise the human mitochondrial proteome are provided, using protein and peptide fractions of biological samples derived from mitochondrial cybrid (cytoplasmic hybrid) cell lines, to identify previously unrecognized mitochondrial molecular components, including modified polypeptides that exhibit structural and/or functional alterations in diseases associated with altered mitochondrial function.