发明名称 |
METHOD FOR HOMOLOGOUS RECOMBINATION USING THE GENES BET AND EXO, BUT NOT GAM, OF THE LAMBDA RECOMBINATION SYSTEM |
摘要 |
A method for replacing a target nucleotide/polynucleotide sequence of a bacterial chromosome with a different nucleotide/polynucleotide sequence, which method comprises: (a) providing a bacterium which is capable of expressing: (1) the gamma genes <i>exo</i> or a functional equivalent thereof and <i>bet</i> or a functional equivalent thereof, but not the gamma gene <i>gam</i> or a functional equivalent thereof; or (2) the gamma gene <i>bet</i> or a functional equivalent thereof and <i>gam</i> or a functional equivalent thereof; (b) providing a polynucleotide construct which comprises: (i) a sequence which corresponds to a first sequence flanking the left hand side of the target sequence; (ii) a second sequence corresponding to a sequence flanking the right hand side of the target sequence; and (iii) positioned between (i) and (ii), the donor sequence; and (c) introducing the polynucleotide construct into the bacterium, thereby to replace the target nucleotide sequence with the sequence different from that of the target nucleotide sequences. The method can be used in the preparation of attenuated bacteria and in the identification of essential genes. |
申请公布号 |
WO02074947(A3) |
申请公布日期 |
2002.12.19 |
申请号 |
WO2002GB01274 |
申请日期 |
2002.03.18 |
申请人 |
ARROW THERAPEUTICS LIMITED;CHARLES, IAN, GEORGE;WHEELER, KERRY |
发明人 |
CHARLES, IAN, GEORGE;WHEELER, KERRY |
分类号 |
C12N1/21;C12N15/10 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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地址 |
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