HUMAN-TYPE ANTI-BLOOD COAGULATION FACTOR VIII ANTIBODY

摘要

A human-type anti-blood coagulation factor VIII (hereinafter referred to as FVIII) which binds to human FVIII and specifically inhibits the blood coagulation activity thereof, and its antibody fragment. An scFv display pha ge library, which has been constructed by using scFv DNA on the basis of random combinations of the VH chain gene and VL chain gene of immunoglobulin originating in lymphocytes of a patient with hemophilia A, is reacted with FVIII having been immobilized on a solid phase via an anti-FVIII monoclonal antibody. Then scFv clones capable of binding to FVIII are screened to there by clarify antibody VH chain and VL chain specific to FVIII.