GENE TRANSFER INTO PRIMATE EMBRYONIC STEM CELLS USGENE TRANSFER INTO PRIMATE EMBRYONIC STEM CELLS USING VSV-G PSEUDOTYPED SIMIAN IMMUNODEFICIENCY VIRUING VSV-G PSEUDOTYPED SIMIAN IMMUNODEFICIENCY VIRUS VECTOR S VECTOR

摘要

A gene is successfully transferred at a high efficiency into primate embryon ic stem cells by using a simian immunodeficiency virus vector (SIV) having been made into the pseudo type with the use of VSV-G protein which is a surface glycoprotein of vesicular stomatitis virus (VSV). Thus, the simian immunodeficiency virus vector for gene transfer into primate embryonic stem cells is provided. Gene transfer into primate embryonic stem cells using the above-described vector is useful in embryological studies, studies on diseases, clinical application, experimental models, etc. in the primates. Moreover, it is useful in assaying and screening genes and reagents for tiss ue- or cell-specific differentiation which are useful in acquiring desired differentiated cells or differentiated tissues from embryonic stem cells.