METHOD OF FORMING STABLE COMPLEX OF TRANSCRIPTION PRODUCT OF DNA ENCODING ARBITRARY PEPTIDE WITH TRANSLATION PRODUCT, NUCLEIC ACID CONSTRUCT TO BE USED IN THE METHOD, COMPLEX FORMED BY THE METHOD, AND SCREENING OF FUNCTIONAL PROTEIN AND MRNA OR DNA ENCODING THE PROTEIN USING THE METHOD

摘要

Using the interaction between an RNA-binding protein and RNA, the interaction between a DNA-binding protein and DNA or a ribosomal inactivated protein, a genotype and a phenotype can be successfully ligated in a stable state in a cell-free system. Moreover, it is found out that a functional protein can be screened by using this stable ligation.