| 摘要 |
<p>A method for determining inorganic pyrophosphate in a sample, which method comprises contacting the sample with an aqueous reagent comprising xanthosine 5'-monophosphate (XMP) or preferrably inosine 5'-monophosphate (IMP), xanthosine phosphoribosyltransferase or preferrably hypoxanthine phosphoribosyltransferase, xanthine oxidase, a divalent cation which is preferrably Mg2+, and a buffering agent which is preferably tris(hydroxymethyl)aminomethane (Tris); and determining production of hydrogen peroxide as a measure or inorganic pyrophosphate in the sample. Preferrably, the reagent further comprises uricase.</p> |
