METHOD F0R THE ISOLATION OF A CLUSTER OF CELLS FROM AN ISOLATED ORGAN

摘要

The invention relates to the field of the isolation of a cluster of cells and more in particular to the isolation of islets of Langerhans. The invention provides a method for the isolation of a cluster of cells from an isolated organ wherein said cluster of cells encompasses a network of vascular tissue. Furthermore the invention provides a method for the isolation and in vitro prolongation of a cluster of cells from an isolated organ wherein said cluster of cells encompasses a network of vascular tissue. The invention also provides a method for prolonging the in vitro lifespan of a cluster of cells comprising culturing said cluster of cells in a culturing medium comprising an extracellular matrix component which surrounds said cluster of cells in vivo.