MUTANT RNA POLYMERASES

摘要

It is intended to provide novel mutant RNA polymerases enabling a transcriptional sequencing method whereby a high SN ratio can be achieved in sequence analysis with the use of capillary and longer and more accurate bas e sequencial data can be obtained by a single reaction. More specifically, a mutant RNA polymerase derived from a wild type RNA polymerase by substitutio n of at least one amino acid and deletion of at least one amino acid, or a mutant RNA polymerase derived from a wild type RNA polymerase by deletion of at least one amino acid, wherein the above-described substitution and/or deletion of amino acid(s) have been performed so that the resultant mutant R NA polymerase has an enhanced ability to incorporate 3~-deoxynucleotide as a substrate compared with the wild type RNA polymerase corresponding thereto.< /SDOAB>