Method for performing multiple recombination events in a genetic system, useful e.g. for removing antibiotic resistance genes, uses mutant recombinase recognition sites

摘要

Use of two non-identical recognition sequence mutants (A) for a sequence-specific recombinase (B) for performing two or more recombination events, mediated by (B), in a single genetic system. Use of two non-identical recognition sequence mutants (A) for a sequence-specific recombinase (B) for performing two or more recombination events, mediated by (B), in a single genetic system. Each (A) has two recognition sequences (RS), separated by a spacer. One RS in each (A) carries mutations that inversely repeated relative to each other, while the other RS has the wild-type sequence. Independent claims are also included for the following: (1) Method for performing multiple (B)-mediated recombination events in a single genetic system using (A); and (2) Six specific nucleic acid sequences that represent loxP mutants with flanking sequences.