发明名称 |
Substrate preparation for chemical-species-specific binding |
摘要 |
A mass biosensor uses an intermediate avidin layer to facilitate binding of a biotinylated antibody to a measurement surface of the biosensor. The avidin layer can be added by the manufacturer of the biosensor, while the biotinylated layer can be added by the user. This two-phase method of chemically modifying the measurement surface significantly reduces the user time required to customize the measurement surface to render it capable of binding selected compounds. An organosilane coupling agent attached to the surface provides sites to which avidin is bound. Avidin acts as a universal receptor of biotinylated compounds with specific binding affinities. Biotinylated antibodies or other biotinylated compounds are added and bind to the immobilized avidin. Surface adsorption is reduced by washing the modified surface with biotin to block potential sites of weak bond formation, electrostatic and hydrophobic interactions.
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申请公布号 |
US2002192718(A1) |
申请公布日期 |
2002.12.19 |
申请号 |
US20010814360 |
申请日期 |
2001.03.21 |
申请人 |
TOM-MOY MAY;MYERHOLTZ CARL ALAN |
发明人 |
TOM-MOY MAY;MYERHOLTZ CARL ALAN |
分类号 |
G01N29/02;G01N33/543;G01N33/552;(IPC1-7):G01N33/53;C12Q1/68 |
主分类号 |
G01N29/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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