The present disclosure relates to method of DNA amplication with a first primer that has a random sequence of nucleotides as its 3' end and a generic sequence 5' of the random nucleotides, as well as a second primer with the generic sequence of the first primer. The disclosure further relates to a method relates to a method of participating DNA on a solid medium to improve DNA amplification. In a preferred embodiment, the presently disclosed methods are used for high-throughput genotyping of DNA samples, such as bloodstains or trace amounts of DNA.
申请公布号
WO02101022(A2)
申请公布日期
2002.12.19
申请号
WO2002US19064
申请日期
2002.06.12
申请人
GENOMICFX, INC.;JI, WAN;GREGG, KEQIN;REUS, BONNIE;KEMPPAINEN, JON;TAYLOR, KRISTEN;DAVIS, SCOTT
发明人
JI, WAN;GREGG, KEQIN;REUS, BONNIE;KEMPPAINEN, JON;TAYLOR, KRISTEN;DAVIS, SCOTT