发明名称 |
Nucleic acid sequence detection employing probes comprising non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents |
摘要 |
Methods and compositions are provided for detecting nucleic acid sequences. Probes comprising a crosslinking agent are combined with a sample which may comprise a target sequence which is complementary to the probe. Hybridization is allowed to occur between complementary sequences. The crosslinking agent is activated. Covalent bonds are formed between the probe and the target sequence if they are hybridized to one another. The crosslinked nucleic acids can then be detected to indicate the presence of the target sequence. Also provided are kits comprising reagents.
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申请公布号 |
US6495676(B1) |
申请公布日期 |
2002.12.17 |
申请号 |
US19990390124 |
申请日期 |
1999.09.03 |
申请人 |
NAXCOR |
发明人 |
WOOD MICHAEL L.;ALBAGLI DAVID;VAN ATTA REUEL B.;THIEN DOUGLAS Y.;CHENG PETER C.;HUAN BINGFANG |
分类号 |
B01L7/00;C07D311/16;C07D407/12;C07F9/655;C07F9/6561;C07H21/00;C12Q1/68;(IPC1-7):C07H21/00 |
主分类号 |
B01L7/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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