发明名称 |
Complex of DNA and microparticle of defatted lipid-binding protein for gene therapy |
摘要 |
Removal of lipid from lipid-bound protein such as human serum albumin to produce defatted protein enhances ability of the protein to bind therapeutic agents for use as a carrier for the therapeutic agent. The defatted protein may be reloaded with cationic and/or anionic lipids such as fatty acids, e.g. DC-Chol, to modify charge, hydrophilicity or hydrophobicity of the defatted protein to further enhance ability of the defatted protein to bind a therapeutic agent. A reloaded lipid itself may be a therapeutic agent. The defatted protein may be produced as microparticles by spray-drying. Defatting can be achieved by removing fatty acids with acidified activated charcoal, or by solvent extraction. A complex of DNA and a microparticle of defatted protein containing a cationic or anionic lipid molecule can be used for gene therapy. Defatted albumin may be reloaded with aminocaprylic acid to provide a microparticle for binding DNA for parenteral delivery. Microparticles of defatted protein having a modified predetermined fatty acid profile may be used as an enhanced ultrasound contrast agent.
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申请公布号 |
US6495527(B1) |
申请公布日期 |
2002.12.17 |
申请号 |
US19990230113 |
申请日期 |
1999.01.22 |
申请人 |
QUADRANT HEALTHCARE, LTD. |
发明人 |
HARRIS ROY;OSBORNE NICHOLAS DAVID |
分类号 |
B82B1/00;A61K9/16;A61K41/00;A61K48/00;A61K49/22;(IPC1-7):A61K31/708;A61K9/14;A61K38/00;C08H1/00;C12N11/02 |
主分类号 |
B82B1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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