MODIFIED MICROPOROUS MEMBRANE FOR NON-SPECIFIC AND SEQUENCE-SPECIFIC NUCLEIC ACID CAPTURE AND METHODS OF USE

摘要

Disclosed herein is a multi-layered (1, 2, 3), composite microporous membrane comprising in at least one layer (1) a highly electropositive hydrophilic material distributed throughout wherein the material is capable of irreversibly binding nucleic acid and, optionally, at least one layer (2) where the material is associated with sequence-specific peptide nucleic acids, permitting the simultaneous or sequential capture, amplification and/or identification of specific nucleic acid sequences of interest. Also disclosed herein are methods of use of the composite membranes of the invention in applications based on the sequence-specific capture and/or amplification and identification of nucleic acid from complex biological samples.