发明名称 |
METHODS AND COMPOSITIONS FOR MUTATION ANALYSIS |
摘要 |
In one aspect, a method for DNA mutation detection including the steps of PCR amplification using preferably Pho DNA polymerase, hybridization, and analysis by denaturing high performance liquid chromatography (DHPLC), the method preferably utilizing a PCR buffer and other solutions that are compatible with DHPLC analysis. In other aspects, compositions and kits including a proofreading DNA polymerase, preferably Pho DNA polymerase, and a DHPLC compatible PCR buffer are provided.
|
申请公布号 |
WO02086444(A9) |
申请公布日期 |
2002.12.12 |
申请号 |
WO2002US12583 |
申请日期 |
2002.04.19 |
申请人 |
TRANSGENOMIC INC. |
发明人 |
GJERDE, DOUGLAS, T.;TAYLOR, PAUL, D.;WALTER, JOANNE;DANIELS, MICHAEL;GRIFFITHS, CAROL;HAEFELE, ROBERT, M.;HANNA, CHRISTOPHER, P. |
分类号 |
C12Q1/68;(IPC1-7):G01N/ |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|