摘要 |
The invention relates to model systems for allergic conditions, and in particular to <i>in vivo</i> model systems in a large animal. The model systems of the invention are especially useful for providing large numbers of activated or non-activated eosinophils, for the discovery and evaluation of novel anti-inflammatory drug targets and for providing a model for the <i>in vivo</i> study of asthma and the effects of allergy treatments. In a preferred embodiment the animal is a sheep.In one embodiment, repeated infusion of house dust mite allergen (HDM) into the mammary gland is used to induce a specific allergic response, which is characterised by the recruitment of inflammatory cells, particularly eosinophils, into the mammary lumen; these cells can be harvested from peripheral blood and mammary lavage (MAL). In a second embodiment, the mammmal is immunised with soluble antigen, for example by repeated subcutaneous immunisation, and then subjected to a single challenge with the same antigen administered directly to the lung.
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申请人 |
MEEUSEN, ELZA, NICOLE, THERESIA;BISCHOF, ROBERT, JUERGEN;SNIBSON, KENNETH, JOHN |
发明人 |
MEEUSEN, ELZA, NICOLE, THERESIA;BISCHOF, ROBERT, JUERGEN;SNIBSON, KENNETH, JOHN |