Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of : (a) optionally reducing disulphide linkages in the polypeptides, if they are present and capping free thiols in the polypeptides, if they are present; (b) contacting a sample comprising one or more polypeptides with a cleavage reagent which cleaves one or more polypeptides on the C-terminal side of a lysine residue to produce peptide fragments; (c) optionally deactivating the cleavage reagent; (d) contacting the sample with a lysine reactive agent to cap epsilon -amino groups; (e) removing those peptides having capped epsilon -amino groups; and (f) recovering the C-terminal peptides.
申请公布号
WO02099436(A2)
申请公布日期
2002.12.12
申请号
WO2002GB02778
申请日期
2002.06.07
申请人
XZILLION GMBH & CO. KG;THOMPSON, ANDREW;HAMON, CHRISTIAN;NEUMANN, THOMAS;PICKERING-BROWN, STUART
发明人
THOMPSON, ANDREW;HAMON, CHRISTIAN;NEUMANN, THOMAS;PICKERING-BROWN, STUART