| 摘要 |
<p>Oligonucleotides such as PNA are used to code for the identity of individual members within a library such that a library present as a mixture can be converted into a spatially addressable format through hybridization to an oligonucleotide array. Such a strategy can greatly facilitate arraying small molecules, antibodies, proteins and oligosaccharides on a chip and allows for binding or other assays to be performed in solution prior to hybridization. This invention is particularly useful for chemical libraries as it allow for combinatorial synthesis employing split and mix technology with positional encoding. The decoding is achieved by hybridization to an oligonucleotide. With high density oligonucleotide arrays, every library member can be analyzed in a highly miniaturized format. As such, this technology readily lends itself to highly miniaturized screening of single or multiple targets simultaneously and profiling.</p> |
