PROCESS OF WRITING OR REWRITING A POLYNUCLEOTIDE SEQUENCE HAVING A PREDETERMINED CONTENT OF CpG DINUCLEOTIDES

摘要

The present invention is concerned with a process for (re)writing a polynucleotide sequence containing a coding sequence for a polypeptide, whereby the content of CpG dinucleotides is adjusted to a predetermined value. These polynucleotides are useful to increase, stabilize, silence and/or reduce gene expression, in particular in protein production, to generate transgenic animal, transgenic plants or to make gene therapy. Preferably, the present invention also relates to process for stably expressing these (re)written polynucleotides in in vivo and in in vivo expression systems.