METHODS FOR LOW BACKGROUND CLONING OF DNA USING LONG OLIGONUCLEOTIDES

摘要

The invention provides methods for the assembly and cloning of target DNAs. Methods for cloning long chemically synthesized oligonucleotides without prior purification are provided. Compromised vectors are used to allow screening or selection for the desired target DNAs. Methods for assembling full-length target DNAs from smaller subsequences are provided, as are methods for purifying oligonucleotides.