| 摘要 |
<p>The present invention relates to a cell capable of producing recombinant human ASA, said cell comprising the DNA fragment shown in SEQ ID NO 1. In particular, the invention relates to a cell comprising the 1578 basepair EcoRI - XbaI fragment of the DNA fragment shown in SEQ ID NO 2. One embodiment of the invention relates to an expression plasmid pAsaExp1 as show in SEQ ID NO 2 for use in the expression of rhASA in cells. Further, the invention relates to a method for the preparation of recombinant human ASA, the method comprising a) introducing, into a suitable vector, a nucleic acid fragment comprising the DNA fragment shown in SEQ ID NO 1, b) transforming a cell with the vector obtained in step a), c) culturing the transformed host cell under conditions facilitating expression of the nucleic acid sequence, d) recovering the expression product from the culture. Optionally, the method may further comprise a fermentation step and/or purification step. The invention further relates to a rhASA produced by the method of the invention and to the use of the rhASA produced for the preparation of a medicament for the treatment of Metachromatic Leukodystrophy.</p> |
