COMPETITIVE ENZYME-LINKED IMMUNOFILTRATION ASSAY USING MULTI-FILTERS AND KIT THEREFOR

摘要

PURPOSE: Provided are competitive enzyme-linked immunofiltration assay using multi-filters and a kit therefor, thereby reducing time and cost for assay. CONSTITUTION: The competitive enzyme-linked immunofiltration assay method comprises the steps of: carrying out immune reaction of a liquid sample to be analyzed by adding an antibody-enzyme complex; removing an immune complex and other immune aggregations by passing a mixed solution containing an immune complex and unreacted antibody-enzyme complex through a membrane filter; carrying out immune reaction of the filtered unreacted antibody-enzyme on an inclusion membrane where the sample to be analyzed is fixed; and analyzing the presence and concentration of the sample to be analyzed by adding an enzyme specific substrate included in the immune complex.