METHOD OF PROVIDING A SUBSTRATE WITH A READY-TO-USE, UNIFORMLY DISTRIBUTED EXTRACELLULAR MATRIX

摘要

Method of providing a substrate with a ready-to-use uniformly distribut ed extracellular matrix is disclosed. This method includes applying extracellul ar matrix components to a substrate area; incubating the extracellular matrix componen ts to allow polymerization thereof; freezing the polymerized extracellular matrix on the substrate area; lyophilizing the frozen extracellular matrix on the substrate area; and allowing the lyophilized extracellular matrix to warm to room temperature. Also contemplated is a cel l culture apparatus having a dried uniformly distributed extracellular matrix formed by the abov e- mentioned method.