| 摘要 |
A method for analyzing biomolecules, such as DNA, mRNA and proteins, in tissue samples, including tissue samples in which these biomolecules are expressed at moderate or low levels of abundance. The method allows multiple tissue samples to be analyzed simultaneously and can be utilized for fresh/frozen tissue samples as well as archival formalin-fixed, paraffin-embedded tissue specimens. The method comprises determining the cellular content of a tissue block, obtaining a plurality of small tissue sections from the tissue block in such a manner that each of said plurality of small tissue section contains at least one cell type, homogenizing each of said plurality of tissue sections in a buffer to create a homogenized specimen, applying said homogenized specimen onto a membrane in order to produce a HistoStamp, applying a labeled probe that is specific for an individual biomolecule corresponding to said at least one cell type to said HistoStamp, and measuring the signal from said labeled probe to determine the abundance level of said biomolecule.
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