| 摘要 |
<p>The invention provides a quantitative hybridization assay for the analysis of mRNA in a target nucleic acid sample. The method comprising the steps of (i) immobilizing the target nucleic acid sample on a solid support; (ii) contacting a labeled antisense probe to a first portion of the said target nucleic acid sample, and a labeled sense probe to a second portion of the said target nucleic acid sample; (iii) detecting and quantitating the signals generated from hybridized antisense probe and hybridized sense probe; and (iv) determining the value represented by the antisense probe signal minus the sense probe signal, said value being proportional to the amount of mRNA in the target nucleic acid sample.</p> |
