| 摘要 |
The present invention provides a process for the preparation of deactivated rice bran lipase by a) extracting lipase enzyme from rice bran and purifying the said lipase enzyme using a salting out agent to obtain active lipase enzyme, b) preparing a ligand in the ratio of 1:10, 1:100, 1:250, 1:750 and 1:1500 mole to mole ratio of protein to ligand; c) mixing the said active lipase enzyme and the ligand and adding to it a substrate, followed by the addition of an activator such as CaCl2 in a concentration of 0.1 M; d) incubating the mixture thus obtained for 4 hours to check for activity, e) separating the deactivated lipase enzyme from the mixture.
|
