Method of agrobacterium mediated plant transformation through treatment of germinating seeds

摘要

The invention relates to a plant transformation method through treating germinating plant seeds with an Agrobacterium strain carrying a foreign DNA fragment . In particular, germinating plant seeds were used as receptors, Ti (tumor inducing) plasmid harbored in an Agrobacterium tumefaciens strain carrying an inserted foreign DNA fragment was used as a gene donor. The germinating plant seeds were co-cultured with the Agrobacterium strain, during the process the foreign DNA fragment was transferred into the donor plant genome. PCR amplification and PCR-Southern hybridization verified that the foreign gene fragment has been transferred into the donor plant and can inherit to the next generation.