| 摘要 |
1,117,948. Extracting medicinal protein. TEIKOKU HORMONE MFG. CO. Ltd. 3 March, 1966 [3 March, 1965], No. 9396/66. Heading A5B. An active material having biological activity with respect to the nervous system is obtained from the submaxillary glands of ungulate mammals, e.g. cattle, horses and swine, by extraction with an aqueous medium e.g. water or 2% saline, precipitating the protein therefrom, redissolving the precipitate and separating the active fraction from the large amount of inactive mucoid present therein. This may be achieved by fractional precipitation of the mucoid by addition of a salt, acid or watermiscible organic solvent or by adding a watersoluble zinc salt which forms a complex with the active material, which may be precipitated free from mucoid by addition of acetone or ethanol. The active material is separable into two fractions e.g. by treating an aqueous saline solution thereof with a weakly basic solution of acrinol, a protein/acrinol complex effective with respect to the proliferation of glial cells is precipitated and a protein effective with respect to the growth of neurons and the regeneration of axons remains in solution. Both may be purified by ion-exchange techniques.
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