ALCOHOL-RESISTANT PROBIOTIC MICROORGANISM AND THE USE THEREOF

摘要

PURPOSE: Provided are an alcohol-resistant probiotic microorganism and the use thereof. The microorganism has high gastric juice and bile acid tolerance, produces beta-galactosidase, so that it can be useful for treatment of lactose intolerance symptom, and can inhibit the infection of Listeria. CONSTITUTION: The alcohol-resistant probiotic microorganism Lactobacillus plantarum MY4(KCCM 10266) is isolated by isolating 100 strains from the Korean feces; separating 28 strains showing similar morphological properties to Lactobacillus sp. from the 100 strains; separating anaerobic gram positive bacillus producing lactic acid from the 28 strains; and separating a strain having high activity of beta-galactosidase and no activity of beta-glucuronidase. The alcohol resistance of Lactobacillus plantarum MY4(KCCM 10266) is examined by culturing Lactobacillus plantarum MY4(KCCM 10266) in an MRS medium at optimal temperature for 12 hours; centrifuging the cultured medium at 6,000 rpm for 10 min to obtain a cell pellet; diluting the cell pellet with a 0.1M phosphate buffer solution; inoculating the diluted cell containing solution into each variable concentration of alcohol containing 0.1M phosphate buffer solution; culturing the solution at 37 deg. C for 4 hours with agitation; and determining the colony forming unit of Lactobacillus plantarum MY4(KCCM 10266) in each cultured solution.