ACETATE-FREE PURIFICATION OF PLASMID DNA ON HYDROXYAPATITE

摘要

Plasmid DNA is purified from a cell lysate by acidifying the lysate with a combination of a mineral acid and an inorganic salt in place of the commonly used acetate buffer, allowing chromosomal DNA and other impurities to precipitate out and recovering the supernatant, and applying the supernatant directly to a hydroxyapatite chromatographic separation medium. The use of t he mineral acid and organic salt avoids the degradation of the hydroxyapatite that frequently accompanies repeated use with acetate and related compounds.