发明名称 |
METHOD FOR DETERMINATION OF GENE FUNCTION |
摘要 |
The present method is useful for the identification of genes, ORF's and other nucleic acid molecules which are essential for the expression of a specific phenotype in microorganisms. The method employs <i>in vitro</i> transposition in conjunction with an chromosomal integration vector containing a specific gene or genetic element whose function is unknown. Subsequent transformation of a recombination proficient host with the vector and growth first under non-integrating conditions and then under integrating conditions, followed by a selection screen for either single or double crossover events results in transformants that may be subjected to phenotypic screens to determine gene function.
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申请公布号 |
WO0171040(A3) |
申请公布日期 |
2002.11.28 |
申请号 |
WO2001US09003 |
申请日期 |
2001.03.21 |
申请人 |
E.I. DUPONT DE NEMOURS AND COMPANY;SHARPE, PAMELA, L.;NAGARAJAN, VASANTHA |
发明人 |
SHARPE, PAMELA, L.;NAGARAJAN, VASANTHA |
分类号 |
C12N15/09;C12N15/10;C12Q1/68;(IPC1-7):C12N15/10 |
主分类号 |
C12N15/09 |
代理机构 |
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