METHOD FOR THE DETERMINATION OF THE CONFLUENCE OF A CELL LAYER ON POROUS BIOMATERIALS

摘要

The invention relates to a novel method, permitting the semiquantitative determination of the confluence of a cell layer on the total surface of a porous biomaterial, without irreversible damage to part or indeed all of the hybrid construct. A method is initially described which can establish the formation of the confluence and the confluence itself, without destroying the hybrid construct and thus permits the finished hybrid construct to be lifted from the essential growing conditions, immediately after the determination of achieving the confluence and to immediately continue with the further use thereof.