An engineered biotin ligase (BirA) includes a chitin binding domain tag and a polyhistidine tag. The engineered BirA may be used to biotinylate proteins such as staphylokinase in a protein purification scheme.
申请公布号
WO02095013(A2)
申请公布日期
2002.11.28
申请号
WO2002CA00760
申请日期
2002.05.24
申请人
UNIVERSITY TECHNOLOGIES INTERNATIONAL, INC.;WONG, SUI-LAM;WU, SAU-CHING