Viral infection of cells

摘要

The present invention relates to an improved in vitro method for infecting cells with a viral vector capable of transporting recombinant nucleic acids into the cells. The method comprises the steps of subjecting the cells to elutriation in a velocity gradient, collecting the cells, contacting the cells with at least one cytokine, and infecting 90% or more of the cells with the viral vector at a multiplicity of infection of 50 to 100.