西红柿斑点枯萎病毒

摘要

本发明描述了西红柿斑点枯萎病毒(TSWV)核蛋白壳的核苷酸序列，和含有TSMV分离株的核蛋白壳核苷酸序列的转基因植物表现出对不同血清型的Tospoviruses具有抗性。另外，生产出含有源自莴苣的TSWV分离株核蛋白壳核苷酸序列的转基因植物，该植物(产生少量的核蛋白壳蛋白)表现出对同源和亲缘关系密切的病毒分离株的抗性；而当植物产生大量核蛋白壳蛋白时，该植物对同源分离株和亲缘关系远的无耐性坏死性斑点病毒(INSV)分离株具有适当水平的保护能力。

主权项

1.选自下列的分离的核苷酸序列：AAATTCTCTT GCAGTGAAAT CTCTGCTCAT GTTAGCAGAA AACAACATCA 50TGCCTAACTC TCAAGCTTTT GTCAAAGCTT CTACTGATTC TAATTTCAAG 100CTGAGCCTCT GGCTAAGGGT TCCAAAGGTT TTGAAGCAGA TTTCCATTCA 150GAAATTGTTC AAGGTTGCAG GAGATGAAAC AAATAAAACA TTTTATTTAT 200CTATTGCCTG CATTCCAAAG CATAACAGTG TTGAGACAGC TTTAAACATT 250ACTGTTATTT GCAAGCATCA GCTCCCAATT CGTAAATGTA AAACTCCTTT 300TGAATTATCA ATGATGTTTT CTGATTTAAA GGAGCCTTAC AACATTATTC 350 ATGATCCTTC ATATCCCCAA AGGATTGTTC ATGCTCTGCT TGAAACTCAC 400ACATCTTTTG CACAAGTTCT TTGCAACAAC TTGCAAGAAG ATGTGATCAT 450CTACACCTTG AACAACCATG AGCTAACTCC TGGAAAGTTA GATTTAGGTG 500AAATAACTTT GAATTACAAT GAAGACGCCT ACAAAAGGAA ATATTTCCTT 550TCAAAAACAC TTGAATGTCT TCCATCTAAC ATACAAACTA TGTCTTATTT 600AGACAGCATC CAAATCCCTT CCTGGAAGAT AGACTTTGCC AGGGGAGAGA 650TTAAAATTTC TCCACAATCT ATTTCAGTTG CAAAATCTTT GTTAAATCTT 700GATTTAAGCG GGATTAAAAA GAAAGAATCT AAGATTAAGG AAGCATATGC 750TTCAGGATCA AAATGATCTT GCTGTGTCCA GCTTTTTCTA ATTATGTTAT 800GTTTATTTTC TTTCTTTACT TATAATTATT TTTCTGTTTG TCATTTCTTT 850CAAATTCCTC CTGTCTAGTA GAAACCATAA AAACAAAAAT AAAAATAAAA 900TAAAATCAAA ATAAAATAAA AATCAAAAAA TGAAATAAAA GCAACAAAAA 950AATTAAAAAA CAAAAAACCA AAAAAGATCC CGAAAGGACA ATTTTGGCCA 1000AATTTGGGGT TTGTTTTTGT TTTTTGTTTT TTTGTTTTTT GTTTTTATTT 1050TTATTTTTAT TTTTATTTTT ATTTTATTTT ATTTTATGTT TTTGTTGTTT 1100TTGTTATTTT GTTATTTATT AAGCACAACA CACAGAAAGCA AACTTTAAT 1150TAAACACACT TATTTAAAAT TTAACACACT AAGCAAGCACA AACAATAAA 1200GATAAAGAAA GCTTTATATA TTTATAGGCT TTTTTATAAT TTAACTTACA 1250GCTGCTTTTA AGCAAGTTCT GTGAGTTTTG CCTGTTTTTT AACCCCAAAC 1300ATTTCATAGA ACTTGTTAAG GGTTTCACTG TAATGTTCCA TAGCAATACT 1350TCCTTTAGCA TTAGGATTGC TGGAGCTAAG TATAGCAGCA TACTCTTTCC 1400CCTTCTTCAC CTGATCTTCA TTCATTTCAA ATGCTTTTCT TTTCAGCACA 1450GTGCAAACTT TTCCTAAGGC TTCCCTGGTG TCATACTTCT TTGGGTCGAT 1500CCCGAGATCC TTGTATTTTG CATCCTGATA TATAGCCAAG ACAACACTGA 1550TCATCTCAAA GCTATCAACT GAAGCAATAA GAGGTAAGCT ACCTCCCAGC 1600ATTATGGCAA GCCTCACAGA CTTTGCATCA TCAAGAGGTA ATCCATAGGC 1650TTGAATCAAA GGGTGGGAAG CAATCTTAGA TTTGATAGTA TTGAGATTCT 1700CAGAATTCC 1709；TTAACACACT AAGCAAGCAC AAACAATAAA GATAAAGAAA GCTTTATATA 50TTTATAGGCT TTTTTATAAT TTAACTTACA GCTGCTTTTA AGCAAGTTCT 100GTGAGTTTTG CCTGTTTTTT AACCCCAAAC ATTTCATAGA ACTTGTTAAG 150GGTTTCACTG TAATGTTCCA TAGCAATACT TCCTTTAGCA TTAGGATTGC 200TGGAGCTAAG TATAGCAGCA TACTCTTTCC CCTTCTTCAC CTGATCTTCA 250TTCATTTCAA ATGCTTTTCT TTTCAGCACA GTGCAAACTT TTCCTAAGGC 300TTCCCTGGTG TCATACTTCT TTGGGTCGAT CCCGAGATCC TTGTATTTTG 350CATCCTGATA TATAGCCAAG ACAACACTGA TCATCTCAAA GCTATCAACT 400GAAGCAATAA GAGGTAAGCT ACCTCCCAGC ATTATGGCAA GCCTCACAGA 450CTTTGCATCA TCAAGAGGTA ATCCATAGGC TTGACTCAAA GGGTGGGAAG 500CAATGTTAGA TTTGATAGTA TTGAGATTCT CAGAATTCCC AGTTTCCTCA 550ACAAGCCTGA CCCTGATCA GCTATCAAGC CTTCTGAAGG TCATGTCAGT 600GGCTCCAATC CTGTCTGAAG TTTTCTTTAT GGTAATTTTA CCAAAAGTAA 650AATCGCTTTG CTTAATAACC TTCATTATGC TCTGACGATT CTTCAGGAAT 700GTCAGACATG AAATAATGCT CATCTTTTTG ATCTGGTCAA GGTTTTCCAG 750ACAAAAAGTC TTGAAGTTGA ATGCTACCAG ATTCTGATCT TCCTCAAACT 800CAAGGTCTTT GCCTTGTGTC AACAAAGCAA CAATGCTTTC CTTAGTGAGC 850TTAACCAT 858；AAATTCTCTT GCAGTGAAAT CTCTGCTCAT GTTAGCAGAA AACAACATCA 50TGCCTAACTC TCAAGCTTTT GTCAAAGCTT CTACTGATTC TAATTTCAAG 100CTGAGCCTCT GGCTAAGGGT TCCAAAGGTT TTGAAGCAGA TTTCCATTCA 150GAAATTGTTC AAGGTTGCAG GAGATGAAAC AAATAAAACA TTTTATTTAT 200CTATTGCCTG CATTCCAAAC CATAACAGTG TTGAGACAGC TTTAAACATT 250ACTGTTATTT GCAAGCATCA GCTCCCAATT CGTAAATGTA AAACTCCTTT 300TGAATTATCA ATGATGTTTT CTGATTTAAA GGAGCCTTAC AACATTATTC 350ATGATCCTTC ATATCCCCAA AGGATTGTTC ATGCTCTGCT TGAAACTCAC 400ACATCTTTTG CACAAGTTCT TTGCAACAAC TTGCAAGAAG ATGTGATCAT 450CTACACCTTG AACAACCATG AGCTAACTCC TGGAAAGTTA GATTTAGGTG 500AAATAACTTT GAATTACAAT GAAGACGCCT ACAAAAGGAA ATATTTCCTT 550TCAAAAACAC TTGAATGTCT TCCATCTAAC ATACAAACTA TGTCTTATTT 600AGACAGCATC CAAATCCCTT CCTGGAAGAT AGACTTTGCC AGGGGAGAAA 650TTAAAATTTC TCCACAATCT ATTTCAGTTG CAAAATCTTT GTTAAATCTT 700GATTTAAGCG GGATTAAAAA GAAAGAATCT AAGATTAAGG AAGCATATGC 750TTCAGGATCA AAATGATCTT GCTGTGTCCA GCTTTTTCTA ATTATGTTAT 800GTTTATTTTC TTTCTTTACT TATAATTATT TTTCTGTTTG TCATTTCTTT 850CAAATTCCTC CTGTCTAGTA GAAACCATAA AAACAAAAAT AAAAATAAAA 900TAAAATCAAA ATAAAATAAA AATCAAAAAA TGAAATAAAA GCAACAAAAA 950AATTAAAAAA CAAAAAACCA AAAAAGATCC CGAAAGGACA ATTTTGGCCA 1000AATTTGGGGT TTGTTTTTGT TTTTTGTTTT TTTGTTTTTT GTTTTTATTT 1050TTATTTTTAT TTTTATTTTT ATTTTATTTT ATTTTATGTT TTTGTTGTTT 1100TTGTTATTTT GTTATTTATT AAGCACAACA CACAGAAAGC AAACTTTAAT 1150TAAACACACT TATTTAAAAT TTAACACACT AAGCAAGCAC AAACAATAAA 1200GATAAAGAAA GCTTTATATA TTTATAGGCT TTTTTATAAT TTAACTTACA 1250GCTGCTTTTA AGCAAGTTCT GTGAGTTTTG CCTGTTTTTT AACCCCAAAC 1300ATTTCATAGA ACTTGTTAAG GGTTTCACTG TAATGTTCCA TAGCAATACT 1350TCCTTTAGCA TTAGGATTGC TGGAGCTAAG TATAGCAGCA TACTCTTTCC 1400CCTTCTTCAC CTGATCTTCA TTCATTTCAA ATGCTTTTCT TTTCAGCACA 1450GTGCAAACTT TTCCTAAGGC TTCCCTGGTG TCATACTTCT TTGGGTCGAT 1500CCCGAGATCC TTGTATTTTG CATCCTGATA TATAGCCAAG ACAACACTGA 1550TCATCTCAAA GCTATCAACT GAAGCAATAA GAGGTAAGCT ACCTCCCAGC 1600ATTATGGCAA GCCTCACAGA CTTTGCATCA TCAAGAGGTA ATCCATAGGC 1650TTGACTCAAA GGGTGGGAAG CAATCTTAGA TTTGATAGTA TTGAGATTCT 1700CAGAATTCCC AGTTTCCTCA ACAAGCCTGA CCCTGATCAA GCTATCAAGC 1750CTTCTGAAGG TCATGTCAGT GGCTCCAATC CTGTCTGAAG TTTTCTTTAT 1800GGTAATTTTA CCAAAAGTAA AATCGCTTTG CTTAATAACC TTCATTATGC 1850TCTGACGATT CTTCAGGAAT GTCAGACATG AAATAATGCT CATCTTTTTG 1900ATCTGGTCAA GGTTTTCCAG ACAAAAAGTC TTGAAGTTGA ATGCTACCAG 1950ATTGTGATCT TCCTCAAACT CAAGGTCTTT GCCTTGTGTC AACAAAGCAA 2000CAATGCTTTC CTTAGTGAGC TTAACCAT 2028； 和AGAGCAATTG GGTCATTTTT TATTCTAAAT CGAACCTCAA CTAGCAAATC 50TCAGAACTGT AATAAGCACA AGAGCACAAG AGCCACAATG TCATCAGGTG 100TTTATGAATC GATCATTCAG ACAAAGGCTT CAGTTTGGGG ATCGACAGCA 150TCTGGTAAGT CCATCGTGGA TTCTTACTGG ATTTATGAGT TTCCAACTGG 200TTCTCCACTG GTTCAAACTC AGTTGTACTC TGATTCGAGG AGCAAAAGTA 250GCTTCGGCTA CACTTCAAAA ATTGGTGATA TTCCTGCTGT AGAGGAGGAA 300ATTTTATCTC AGAACGTTCA TATCCCAGTG TTTGATGATA TTGATTTCAG 350CATCAATATC AATGATTCTT TCTTGGCAAT TTCTGTTTGT TCCAACACAG 400TTAACACCAA TGGAGTGAAG CATCAGGGTC ATCTTAAAGT TCTTTCTCTT 450GCCCAATTGC ATCCCTTTGA ACCTGTGATG AGCAGGTCAG AGATTGCTAG 500CAGATTCCGG CTCCAAGAAG AAGATATAAT TCCTGATGAC AAATATATAT 550CTGCTGCTAA CAAGGGATCT CTCTCCTGTG TCAAAGAACA TACTTACAAA 600GTCGAAATGA GCCACAATCA GGCTTTAGGC AAAGTGAATG TTCTTTCTCC 650TAACAGAAAT GTTCATGAGT GGCTGTATAG TTTCAAACCA AATTTCAACC 700AGATCGAAAG TAATAACAGA ACTGTAAATT CTCTTGCAGT CAAATCTTTG 750CTCATGGCTA CAGAAAACAA CATTATGCCT AACTCTCAAG CTTTTGTTAA 800AGCTTCTACT GATTCTCATT TTAAGTTGAG CCTTTGGCTG AGAATTCCAA 850AAGTTTTGAA GCAAATAGCC ATACAGAAGC TCTTCAAGTT TGCAGGAGAC 900GAAACCGGTA AAAGTTTCTA TTTGTCTATT GCATGCATCC CAAATCACAA 950CAGTGTGGAA ACAGCTTTAA ATGTCACTGT TATATGTAGA CATCAGCTTC 1000CAATCCCTAA GTCCAAAGCT CCTTTTGAAT TATCAATGAT TTTCTCCGAT 1050CTGAAAGAGC CTTACAACAC TGTGCATGAT CCTTCATATC CTCAAAGGAT 1100TGTTCATGCT TTGCTTGAGA CTCACACTTC CTTTGCACAA GTTCTCTGCA 1150ACAAGCTGCA AGAAGATGTG ATCATATATA CTATAAACAG CCCTGAACTA 1200ACCCCAGCTA AGCTGGATCT AGGTGAAAGA ACCTTGAACT ACAGTGAAGA 1250TGCTTCGAAG AAGAAGTATT TTCTTTCAAA AACACTCGAA TGCTTGCCAG 1300TAAATGTGCA GACTATGTCT TATTTGGATA GCATCCAGAT TCCTTCATGG 1350AAGATAGACT TTGCCAGAGG AGAGATCAGA ATCTCCCCTC AATCTACTCC 1400TATTGCAAGA TCTTTGCTCA AGCTGGATTT GAGCAAGATC AAGGAAAAGA 1450AGTCCTTGAC TTGGGAAACA TCCAGCTAGT ATCTAGAATA AAAGTGGCTC 1500ATACTACTCT AAGTAGTATT TGTCAACTTG CTTATCCTTT ATGTTGTTTA 1550TTTCTTTTAA ATCTAAAGTA AGTTAGATTC AAGTAGTTTA GTATGCTATA 1600GCATTATTAC AAAAAATACA AAAAAATACA AAAAAATACA AAAAATATAA 1650AAAACCCAAA AAGATCCCAA AAGGGACGAT TTGGTTGATT TACTCTGTTT 1700TAGGCTTATC TAAGCTGCTT TTGTTTGAGC AAAATAACAT TGTAACATGC 1750AATAACTGGA ATTTAAAGTC CTAAAAGAAG TTTCAAAGGA CAGCTTAGCC 1800AAAATTGGTT TTTGTTTTTG TTTTTTTGTT TTTTGTTTTT TTGTTTTATT 1850TTTATTTTTA GTTTATTTTT TGTTTTTGTT ATTTTTATTT TTATTTTATT 1900TTCTTTTATT TTATTTATAT ATATATCAAA CACAATCCAC ACAAATAATT 1950TTAATTTCAA ACATTCTACT GATTTAACAC ACTTAGCCTG ACTTTATCAC 2000ACTTAACACG CTTAGTTAGG CTTTAACACA CTGAACTGAA TTAAAACACA 2050CTTAGTATTA TGCATCTCTT AATTAACACA CTTTAATAAT ATGCATCTCT 2100GAATCAGCCT TAAAGAAGCT TTTATGCAAC ACCAGCAATC TTGGCCTCTT 2150TCTTAACTCC AAACATTTCA TAGAATTTGT CAAGATTATC ACTGTAATAG 2200TCCATAGCAA TGCTTCCCTT AGCATTGGGA TTGCAAGAAC TAAGTATCTT 2250GGCATATTCT TTCCCTTTGT TTATCTGTGC ATCATCCATT GTAAATCCTT 2300TGCTTTTAAG CACTGTGCAA ACCTTCCCCA GAGCTTCCTT AGTGTTGTAC 2350TTAGTTGGTT CAATCCCTAA CTCCTTGTAC TTTGCATCTT GATATATGGC 2400AAGAACAACA CTGATCATCT CGAAGCTGTC AACAGAAGCA ATGAGAGGGA 2450TACTACCTCC AAGCATTATA GCAAGTCTCA CAGATTTTGC ATCTGCCAGA 2500GGCAGCCCGT AAGCTTGGAC CAAAGGGTGG GAGGCAATTT TTGCTTTGAT 2550AATAGCAAGA TTCTCATTGT TTGCAGTCTC TTCTATGAGC TTCACTCTTA 2600TCATGCTATC AAGCCTCCTG AAAGTCATAT CCTTAGCTCC AACTCTTTCA 2650GAATTTTTCT TTATCGTGAC CTTACCAAAA GTAAAATCAC TTTGGTTCAC 2700AACTTTCATA ATGCCTTGGC GATTCTTCAA GAAAGTCAAA CATGAAGTGA 2750TACTCATTTT CTTAATCAGG TCAAGATTTT CCTGACAGAA AGTCTTAAAG 2800TTGAATGCGA CCTGGTTCTG GTCTTCTTCA AACTCAACAT CTGCAGATTG 2850AGTTAAAAGA GAGACAATGT TTTCTTTTGT GAGCTTGACC TTAGACATGG 2900TGGCAGTTTA GATCTAGACC TTTCTCGAGA GATAAGATTC AAGGTGAGAA 2950AGTGCAACAC TGTAGACCGC GGTCGTTACT TATCCTGTTA ATGTGATGAT 3000TTGTATTGCT GAGTATTAGG TTTTTGAATA AAATTGACAC AATTGCTCT 3049